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microrna microarray profiling system  (Agilent technologies)


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    Agilent technologies microrna microarray profiling system
    Microrna Microarray Profiling System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microrna+microarray+profiling+system/pm29170044-420-1-0
    Average 90 stars, based on 1 article reviews
    microrna microarray profiling system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: RNA (1 μl) was used for quantitative analysis using a NanoDrop ND-2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and an Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, CA, USA) was used to examine RNA integrity. .. Detection of serum miRNA expression using the Agilent MicroRNA Microarray Profiling System Total RNA (100 ng) was extracted from the sera of 9 ESCC patients and 9 healthy volunteers, and used for dephosphorylation, Cy3 labeling and hybridization with a miRNA microarray using the miRNA Complete Labeling and Hyb kit (Agilent Technologies Inc.), according to the manufacturer's instructions. .. Following hybridization, the microarray was washed with the washing buffer in the Gene Expression Wash Buffer kit (Agilent Technologies Inc..

    Microarray:

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: RNA (1 μl) was used for quantitative analysis using a NanoDrop ND-2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and an Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, CA, USA) was used to examine RNA integrity. .. Detection of serum miRNA expression using the Agilent MicroRNA Microarray Profiling System Total RNA (100 ng) was extracted from the sera of 9 ESCC patients and 9 healthy volunteers, and used for dephosphorylation, Cy3 labeling and hybridization with a miRNA microarray using the miRNA Complete Labeling and Hyb kit (Agilent Technologies Inc.), according to the manufacturer's instructions. .. Following hybridization, the microarray was washed with the washing buffer in the Gene Expression Wash Buffer kit (Agilent Technologies Inc..

    Article Title: Dual Suppressive Effect of miR-34a on the FOXM1/eEF2-Kinase Axis Regulates Triple-Negative Breast Cancer Growth and Invasion
    Article Snippet: .. & Fulmer-Smentek, S. Agilent microRNA microarray profiling system. ..

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: .. Therefore, in the present study, the Agilent MicroRNA Microarray Profiling System and bioinformatics methods were used for the analysis and identification of specific miRNAs in the serum of ESCC patients. ..

    Article Title: MicroRNA expression profiling in blood from fragile X-associated tremor/ataxia syndrome patients.
    Article Snippet: .. D'ANDRADE, P. N. & FULMER-SMENTEK, S. (2012) Agilent microRNA microarray profiling system. ..

    Article Title: Differential gene expression in Mycobacterium bovis challenged monocyte-derived macrophages of cattle.
    Article Snippet: Accepted Manuscript Differential gene expression in Mycobacterium bovis challenged monocyte-derived macrophages of cattle Sanjeev Kumar Shukla, Shubhra Shukla, Anuj Chauhan, Sarvjeet, Rehan Khan, Lakshya Veer Singh, Naveen Sharma, Chandan Prakash, Ajay Vir Singh, Manjit Panigrahi PII: S0882-4010(17)31235-4 DOI: 10.1016/j.micpath.2017.11.030 Reference: YMPAT 2597 To appear in: Microbial Pathogenesis Received Date: 27 September 2017 Revised Date: 14 November 2017 Accepted Date: 18 November 2017 Please cite this article as: Shukla SK, Shukla S, Chauhan A, Sarvjeet , Khan R, Singh LV, Sharma N, Prakash C, Singh AV, Panigrahi M, Differential gene expression in Mycobacterium bovis challenged monocyte-derived macrophages of cattle, Microbial Pathogenesis (2017), doi: 10.1016/ j.micpath.2017.11.030.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

    De-Phosphorylation Assay:

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: RNA (1 μl) was used for quantitative analysis using a NanoDrop ND-2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and an Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, CA, USA) was used to examine RNA integrity. .. Detection of serum miRNA expression using the Agilent MicroRNA Microarray Profiling System Total RNA (100 ng) was extracted from the sera of 9 ESCC patients and 9 healthy volunteers, and used for dephosphorylation, Cy3 labeling and hybridization with a miRNA microarray using the miRNA Complete Labeling and Hyb kit (Agilent Technologies Inc.), according to the manufacturer's instructions. .. Following hybridization, the microarray was washed with the washing buffer in the Gene Expression Wash Buffer kit (Agilent Technologies Inc..

    Labeling:

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: RNA (1 μl) was used for quantitative analysis using a NanoDrop ND-2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and an Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, CA, USA) was used to examine RNA integrity. .. Detection of serum miRNA expression using the Agilent MicroRNA Microarray Profiling System Total RNA (100 ng) was extracted from the sera of 9 ESCC patients and 9 healthy volunteers, and used for dephosphorylation, Cy3 labeling and hybridization with a miRNA microarray using the miRNA Complete Labeling and Hyb kit (Agilent Technologies Inc.), according to the manufacturer's instructions. .. Following hybridization, the microarray was washed with the washing buffer in the Gene Expression Wash Buffer kit (Agilent Technologies Inc..

    Hybridization:

    Article Title: Serum miRNA expression in patients with esophageal squamous cell carcinoma
    Article Snippet: RNA (1 μl) was used for quantitative analysis using a NanoDrop ND-2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA), and an Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Santa Clara, CA, USA) was used to examine RNA integrity. .. Detection of serum miRNA expression using the Agilent MicroRNA Microarray Profiling System Total RNA (100 ng) was extracted from the sera of 9 ESCC patients and 9 healthy volunteers, and used for dephosphorylation, Cy3 labeling and hybridization with a miRNA microarray using the miRNA Complete Labeling and Hyb kit (Agilent Technologies Inc.), according to the manufacturer's instructions. .. Following hybridization, the microarray was washed with the washing buffer in the Gene Expression Wash Buffer kit (Agilent Technologies Inc..



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    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The <t>microRNA</t> profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
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    a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Journal: Cell Death & Disease

    Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

    doi: 10.1038/s41419-024-06744-0

    Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

    Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

    Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

    microRNA and gene expression microarray datasets related to idiopathic pulmonary fibrosis.

    Journal: Mediators of Inflammation

    Article Title: Analysis of Microarray-Identified Genes and MicroRNAs Associated with Idiopathic Pulmonary Fibrosis

    doi: 10.1155/2017/1804240

    Figure Lengend Snippet: microRNA and gene expression microarray datasets related to idiopathic pulmonary fibrosis.

    Article Snippet: Microarray data from IPF-related microRNA and mRNA expression profiles were retrieved and downloaded from the National Center for Biotechnology Information (NCBI) GEO database ( http://www.ncbi.nlmNih.gov/geo ).

    Techniques: Expressing, Microarray